The Effect of Pepper Nigrum, Olea Europaea Mouthwash with Non Surgical Periodontal Therapy on Clinical Outcome And Oxidative Stress in Chronic Periodontitis Patients

Abstract

Introduction: gargles are liquid topical drugs  that action  by gargling and  rinsing into mouth. Many  mouthwashes are  existences   and by prescription with a broad  level  of active components  which can be manipulated as a  helper  to aid in the multifactorial therapy of complicated  oral situation ,  consisting  of gingivitis, halitosis, and  periodontal illness. Newly ( Pepper Nigrum  , Olea Europaea )  are herbal  with   antibacterial, anti-inflammatory, antioxidant and antifungal influences, using plant extracts as mouthwash , in addition to being safe and inexpensive. Aim :To measure  the effect of herbal mouthwash (Pepper Nigrum , Olea Europaea) and non-surgical periodontal therapy  in comparison with periodontal therapy alone  on oxidative stress , total antioxidant capacity , antioxidant  enzyme activities in all spit of sick person with chronic periodontitis. To investigate association amidst oxidative stress parameter, antioxidant enzymes, total antioxidant capacity and  periodontal parameter Material  and ways : A flow up study was conducted on a randomly selected sample of 72 patients (39male and 33femal) aged 25-55 years from patients  attending college of dentistry ,department of periodontology from December 2020, to April  2020 .participants  were assigned into three categories  , every catigory consist of 24 patients : first group receiving non-surgical periodontal treatment NSPT and Pepper Nigrum  mouthwash, second groups receiving Olea Europaea  and NSPT  while the third group control receiving NSPT only. Clinical attachment loss (CAL) and  Plaque Index (PI) were recorded. Salivary oxidative stress eight hydroxy  deoxyguanosine (8-OHdG),salivary  Catalase(CAT) and salivary Total antioxidant capacity (TAOC), were measured Result : The concentration of salivary antioxidants (CAT)  significantly increase p-value <0.006, the periodontal parameter (CAL , PI) and oxidative stress 8-OHdG significantly decrease p-value   <0.0001  and  <0.001 respectively after two weeks following the NSPT  in group of  Pepper Nigrum, while TAOC was not changed .Moreover, for Olea Europaea  group the clinical parameters of CAL, PI showed a significant decrease  and p-value was (P<0.0001) .Also  CAT   and TAOC express  a significant increase  (P<0.0001) .In addition to oxidative stress 8-OHdG marker showed a significant decrease (p <0.0001)  Conclusion: According to our research, Pepper Nigrum, Olea Europaea mouthwash with NSPT reduced  OS and bettering  periodontal status and elevated salivary antioxidant  . So, it is advised that Pepper Nigrum  , Olea Europaea  mouthwash   with NSPT could  be further efficient in control of inflammation in sick people with CP positive and negative correlations were found  between biochemical and clinical parameters in Pepper Nigrum group  while more positive correlation was  found in Olea Europaea  group. It indicates that periodontitis can be one of the factors for decreased antioxidant level in our body. It indicates that periodontitis can be one of the factors for decreased antioxidant level in our body