The effect of papain enzyme in various doses to the growth and intestinal of cantang grouper (Epinephelus fuscogutattus lanceolotus)

Abstract

This research was carried out to observe the appearance of the cantang grouper (Ephinephelus fuscogutattus lanceolotus) intestine which was fed with exogenous papain enzyme-added, and the fish were raised in the cage on the coast of Pangandaran, West Java Province from January to March 2019. A completely Randomized Design Method in this study was performed with five treatments and three repetitions with enzyme doses of 0%, 1.25%, 2.5%, 3.75%, and 5% for 60 days. The fish used were two months old with an average weight 20 ± 10 g and randomly placed in fifteen cages with the size of 1m × 1m × 1.2m. All fish were fed commercial diets (CP: 40%) at a feeding level of 5% sampling was carried out every 10 days for 60 days. The Average Daily Gain (ADG) calculation was done by calculating the initial and final weights, the fish were dissected and the intestine conditions was carried out by taking the digestive tract from the anterior to the posterior. The parameters observed were ADG and intestine conditions (number of villi, villi length, necrosis, and goblet cells) in the region of anterior, midterior, and posterior. The results showed that the ADG was significantly different in all treatments, the best was the addition of enzymes 3.75% and 5 % namely 2.26 and 2.30 gram/day. The number of villi at the anterior intestine was 32, the length of villi was 290.34 μm, necrosis cells was 39 and goblet was 168. Meanwhile, at the midterior the number of villi was 26, the length of villi was 275.18 μm, necrosis cells was 47 and goblet was 177. The number of villi at the posterior was 28, the length of villi was 277.26 μm, Necrosis cells was 56 and goblet was 201. Overall it can be concluded that the feed was added with papain enzyme of 3.75 % shows a significant effect on the growth and intestinal conditions of the Cantang grouper.