Abstract
The research is aimed to study the effect of paclitaxel on the viability of U14 cell line of cervical cancer, and to provide new ideas for further exploring the mechanism of paclitaxel chemotherapy to cervical cancer. Then, different concentrations of paclitaxel were used to treat U14 cells of cervical cancer in logarithmic phase growth. After culturing these cells for 24h, 48h or 72h, MTT method and automatic enzyme-linked immunosorbent assay system were used to evaluate the survival rate of cultured cells with different concentrations of paclitaxel. These results showed that paclitaxel has a significant inhibitory effect on the proliferation of U14 cells in a concentration- and time-dependent manner, compared with the control group. Paclitaxel concentration of the IC50 of U14 cells was 168.8μg/ml at 24h, 22. 15 μg/ml at 48h and 8. 04μg/ml at 72 h. As the time increased, the IC50 value of U14 cells gradually decreased. The results of linear regression analysis are as follows: 24 h, y=-0.005x + 1.344, R<sup>2</sup>=0.779; 48 h, y=-0.013x +0.788, R<sup>2</sup>=0.923; 72 h, y=-0.056x + 0.950, R<sup>2</sup>=0.908. The dose-effect curve of paclitaxel on U14 cells for 48 h and the trend of linear regression fitting are better, and the growth inhibition of cells shows a clear relationship between time and dose. In conclusion, paclitaxel has an obvious inhibitory effect on the activity of cervical cancer U14 cells in mice, which provides new ideas for further exploring the mechanism of cervical cancer paclitaxel chemotherapy.
Highlights
Cervical cancer is the second highest incidence of malignant tumors in women and the third leading cause of death of women with malignant tumors [1]
The effect of paclitaxel on U14 cells for 48 h was better with the trend of linear regression fitting, and the growth inhibition of the cells shows a significant time-effect and dose-effect relationship (Figures 1, 2)
The IC50 value of 48 h was 22.15 μg/ml which was suit for subsequent experiments that exploring the effect of paclitaxel on U14 cells
Summary
Cervical cancer is the second highest incidence of malignant tumors in women and the third leading cause of death of women with malignant tumors [1]. The main treatment methods for cervical cancer are surgery, radiation therapy and chemotherapy, but the survival rate of patients has not been significantly improved [3, 4] Its main anti-tumor mechanism is to promote the polymerization of tubulin dimer and stabilize the microtubules, thereby inhibiting the normal dynamic reorganization of the microtubule network, which is important for the function of interphase and mitotic cells [6]. It leads to the abnormal arrangement of microtubule bundles, affecting tumor cell division [7]
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