The effect of p-chlorophenylalanine on the development of amphibian notochord: [formula omitted], Department of Biology, University of Turku, SF-20500 Turku, Finland

Abstract

By means of micro-scale chromatography, histones from different regions of newt embryos in the course of development were analyzed qualitatively and quantitatively.The first appearance of lysine-rich histones occurred at the animal-ventral side of the blastula. These increased rapidly in the ectodermal regions at middle gastrula. The time of appearance of lysine-rich histones in the other regions, i. e. mesoderm and endoderm, was delayed.Arginine-rich histone, being a major component in blastula, tended to decrease in every region as development proceeded, though it was predominant in the endoderm throughout early embryogenesis.The pattern of histone subcomponents tended to converge toward a characteristic type, and the histone content in every region approached the DNA content toward the end of early embryogenesis.