The Effect of Oxygen Deprivation on Inorganic Nitrogen Uptake in an Antarctic Macrolichen

Abstract

Snow meltwater containing 36 ng ml−1 NO3−−N (raised here to between 95–101 ng ml−1 NO3−−N) and 112 ng ml−1 NH4+ −N was sprayed onto illuminated Usnea sphacelata at 2°C in a 2−1 capacity transparent perspex chamber force-ventilated with either air or O2− (and CO2−) free N2. The NO3-concentration in meltwater recirculated through a layer of U. sphacelata fell to c. 8 ng ml−1 after 1.25 h. Although the pattern of decline was broadly comparable in both air and N2, the initial rate of decline was lower in N2. When undepleted meltwater was continuously sprayed onto the lichen and the effluent collected for analysis, the lichen was found to retain 55% of the wet deposited NO3− in air but only 27% under N2. Up to 90%) of NH4+ supplied in a continuous spray of meltwater was retained by the lichen but this was affected little by O2 and CO2 deprivation.