Abstract
Work on the thromboplastic activity of human platelets necessitated the preparation of platelet concentrates. As a preliminary step, platelet-rich plasma was obtained by slow centrifugation of oxalated or citrated whole blood. Subsequently, it was observed that the supernatant plasma of citrated blood appeared more opalescent and yielded a larger platelet mass than did that derived from oxalate blood. Although citrate and oxalate are commonly used in collecting blood for coagulation studies, no information on such a difference was found in the available literature. More precise quantitative work was necessary to confirm this finding. The investigation described in this communication was performed to decide whether citrate was superior to oxalate for the purpose of separating the maximal numbers of platelets from blood collected into these two anticoagulants.
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