Abstract
The uptake by liver slices of radioactive acetate, palmitate, stearate, linoleate and glycerol into glycerolipids was compared in fed and fasted (overnight, 16 hr) rats.The incorporation of l-14C-acetate into long-chain fatty acids and glycerolipids was depressed by fasting. There was a considerable decrease in the incorporation of 1-14C-palmitate into triglyceride (TG) and that of l-14C-stearate into phosphatidylcholine (PC) in fasted liver slices. No such differences were observed with l-14C-linoleate. The incorporation of l-14C-glycerol into TG was slightly decreased, whereas that into PC and phosphatidylethanolamine (PE) was increased by fasting.These observations, together with those with the incorporation of the precursors into molecular species of TG, PC and PE, suggested that the changes in the fatty acid composition of glycerolipids by fasting may be governed by the changes in the availability of acyl moieties as well as in the relative balance of the pathways participating to formation of TG and...
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