Abstract
There are sufficient evidences that Lewis antigens are tumor-associated molecules and correlated to high grade and poor prognosis tumors. In this study, we investigated the effect of (α1,3)-fucosyltransferase VII overexpression on the synthesis of sLex and adhesive capability of human colon carcinoma HT-29 cells to HUVECs.The pIRES2-EGFP-FucT VII eukaryotic expression vector were transiently transfected into HT-29 cells. The changes of FucT VII protein and mRNA expression were determined by flow cytomet- ry and Real-Time PCR; the effect of FucT VII overexpression on synthesis of its downstream product-sLex is detected by Flow cytometry; Rose-Bengal method is used to assay the capability of HT-29 cell adhesion to HUVECs. Results: Eukaryotic expression vector pIRES2-EGFP-FucT VII was successfully transfected into HT-29 cells and made FucT VII overexpressed; compared with that of control group, expression level of the sLeX on the surface of FucT VII transfected HT-29 cells was significantly higher; FucT VII overexpression could enhance the adhesive capability of HT-29 cells to HUVECs. Our data suggest that overexpression of FucT VII could strengthen adhesion of sLeX-mediated HT-29 cells to HUVECs through upregulating sLeX synthesis.
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