Abstract
Objective Compare the effects of ageing and ovariectomy on biomarkers of urogenital ageing in old and young-adult rats. Methods Fisher 344 rats (18- and 3-months-old, n=6×2) underwent ovariectomy. Age-matched sham animals received no intervention (n=6×2). One month later, biomarkers of urogenital ageing were evaluated (light microscopic count of urethral and anal canal submucosal blood vessels, Western blot analysis of urethral and anal canal submucosal collagen I and III and cytoplasmic p27kip1 expression in the striated urethral and anal sphincters and levator ani and gel electrophoresis of isomyosin I proportion in these muscles) and compared in all groups (n=24). Results All biomarkers of urogenital ageing studied were significantly increased in old compared to young-adult sham rats. Ovariectomy significantly increased these changes further in old versus young-adult rats with either smaller or larger differential effect than ageing compared to young-adult sham animals. Conclusion Ovariectomy significantly increases the expression of urogenital ageing markers in old versus young-adult rats suggesting an independent and synergistic deleterious effect of hypoestrogenism and ageing. Ovariectomy significantly exacerbates normative urogenital ageing changes in rats.
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