Abstract
The present study was aimed to evaluate the effect of naturally occurring organosulfur compounds namely allyl isothiocyanate (AITC) and benzyl isothiocyanate (BITC) on peroxynitrite-induced cytotoxicity in milk-derived polymorphonuclear neutrophils (PMNs), utilizing the tetrazolium salt XTT assay to measure cell survival. 3-Morpholinsydnonimine (SIN-1), a peroxynitrite donor, induced the loss of PMNs viability in a concentration-dependent manner with estimated EC50 value of 375 microM. Pretreatment with AITC or BITC further enhanced cytotoxicity in SIN-1-treated cells; however, AITC or BITC alone at concentration ≤3 microM did not cause the cytotoxic effect as compared to untreated control cells. On the contrary, glutathione (GSH), melatonin and uric acid markedly and concentration dependently attenuated the cytotoxic effect of SIN-1. These data suggest that these organosulfur compounds could render cells more susceptible to injury and death mediated by oxidants. This work was supported by the Commission of Higher Education, Ministry of Education and the Thailand Research Fund.
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