Abstract
Cell manipulation using optically induced dielectrophoresis (ODEP) in microfluidic systems has attracted the interest of scientists due to its simplicity. Although this technique has been successfully demonstrated for various applications, one fundamental issue has to be addressed—Whether, the ODEP field affects the native properties of cells. To address this issue, we explored the effect of ODEP electrical conditions on cellular properties. Within the experimental conditions tested, the ODEP-based cell manipulation with the largest velocity occurred at 10 Vpp and 1 MHz, for the two cancer cell types explored. Under this operating condition, however, the cell viability of cancer cells was significantly affected (e.g., 70.5 ± 10.0% and 50.6 ± 9.2% reduction for the PC-3 and SK-BR-3 cancer cells, respectively). Conversely, the exposure of cancer cells to the ODEP electrical conditions of 7–10 Vpp and 3–5 MHz did not significantly alter the cell viability, cell metabolic activity, and the EpCAM, VIM, and ABCC1 gene expression of cancer cells. Overall, this study fundamentally investigated the effect of ODEP electrical conditions on the cellular properties of cancer cells. The information obtained is crucially important for the utilization of ODEP-based cell manipulation in a microscale system for various applications.
Highlights
The fine manipulation of biological cells has attracted considerable interests in a wide variety of biomedical studies
30.6 μm s−1 and 100.0 ± 20.0 μm s−1 for the PC-3 and SK-BR-3 cancer cells, respectively) of a light image that can manipulate cells occurred at the voltage magnitude and frequency of 10 Vpp and
This study fundamentally investigated the effect of optically induced dielectrophoresis (ODEP) electrical conditions on the cellular properties of cancer cells
Summary
The fine manipulation of biological cells has attracted considerable interests in a wide variety of biomedical studies (e.g., cell sorting [1,2,3,4,5,6,7,8], the isolation and purification of cells [1,2,3,4,5,6,7,8,9], cell patterning [10,11,12,13,14], tissue engineering [10,11,14], or various cell-based bioassays [12,13]). To tackle the technical issues, the optically induced dielectrophoresis (ODEP)-based technique could open up a new horizon for the manipulation of microparticles in a more efficient, flexible, and user-friendly manner [23,24]
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