The Effect of On-Pump and Off-Pump Bypass Operations on Oxidative Damage and Antioxidant Parameters

Fevzi Sarper Turker,Ayse Dogan

doi:10.1155/2017/8271376

Fevzi Sarper Turker, Ayse Dogan

Open Access

https://doi.org/10.1155/2017/8271376

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Abstract

Objective The aim of the study is to determine the oxidative status in on-pump and off-pump coronary artery surgery and contribute to possible surgical choices in clinical practices in accordance with the information obtained as a result of this study. Methods 52 patients undergoing open heart surgery (26 patients in on-pump group and 26 patients in off-pump group) were included in the study. MDA, GPx, GSH, CAT, and SOD were investigated in blood samples. Results In the on-pump group, it was determined that there were a significant increase in MDA level in the peroperative period compared to the preoperative and postoperative periods and a significant increase in GSH level in the postoperative period than in the preoperative period. Additionally, while there was a significant decrease in CAT activity in the postoperative period than in the peroperative period, there was a statistically significant increase in SOD enzyme activity in the postoperative period compared to the preoperative and peroperative periods. A statistically significant increase was observed in SOD enzyme activity in the postoperative period in on-pump compared to off-pump group. Conclusion It is thought that this oxidative damage can be suppressed by administering a suitable antioxidant supplement in the preoperative and peroperative periods among patients undergoing the on-pump operation.

Highlights

Free radicals are relatively reactive molecules derived from oxygen, nitrogen, or sulphur molecules
While there was a significant decrease in CAT activity in the on-pump group in the postoperative period compared to the peroperative period, a statistically significant increase was determined in the superoxide dismutase (SOD) enzyme activity in the postoperative period compared to the preoperative and peroperative periods (Table 1, Figures 1–4)
A significant increase was determined in MDA level in the preoperative and peroperative periods in the on-pump group compared to the off-pump group, and no significant difference was observed in the postoperative period

Summary

Introduction

Free radicals are relatively reactive molecules derived from oxygen, nitrogen, or sulphur molecules. Since these radicals are usually derived from oxygen, these molecules are called as reactive oxygen molecules (reactive oxygen species/ROS). ROS is the products of normal metabolism such as aerobic metabolism in mitochondria in ATP production. Being generated under normal conditions, ROS is scavenged by the cells’ own defense mechanisms against. Enzymatic and free radical scavenging activities are among these defense mechanisms. The balance between ROS production and the defense mechanisms against ROS prevents the cell injury. ROS levels may significantly increase and as a result, the injury may be observed in numerous cellular molecules such as lipids, proteins, and DNA hydrogen peroxide (H2O2),

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