The Effect of Omnaris® on the Inflammatory Reaction and Mucin Gene Expression from Nasal Polyp Epithelial Cells

Abstract

Received December 13, 2013 Revised May 12, 2014 Accepted June 9, 2014 Address for correspondence Seung-Heon Shin, MD Department of OtolaryngologyHead and Neck Surgery, School of Medicine, Catholic University of Daegu, 33 Duryugongwon-ro 17-gil, Nam-gu, Daegu 705-718, Korea Tel +82-53-650-4530 Fax +82-53-650-4533 E-mail hsseung@cu.ac.kr Background and ObjectivesZZGlucocorticoids are a potent anti-inflammatory agent. The authors conducted this study to investigate the effect of Omnaris on suppression of inflammation induction and mucin gene expression in nasal polyp epithelial cells. Subjects and MethodZZPrimary nasal polyp epithelial cells were stimulated by 5 ug/mL of streptococcal enterotoxin B (SEB) and lipopolysaccharide (LPS). To determine the effects of Omnaris, cells were pretreated with 200, 100, 10, 1 ng/mL of Omnaris. The anti-inflammatory effect of epithelial cells were confirmed by measuring interleukin (IL)-6, IL-8, and granulocyte-macrophage colony stimulating factor (GM-CSF), and mucin gene expressions were determined by real time PCR for MUC4, MUC5AC, MUC5B, MUC8. ResultsZZSEB and LPS enhanced the production of IL-6, IL-8, and GM-CSF from nasal polyp epithelial cells. The increased cytokine levels were significantly suppressed by Omnaris at 100 and 10 ng/mL. The expressions of MUC4, MUC5AC, MUC5B, MUC8 mRNA, and MUC4 mRNA were increased by SEB and LPS, respectively. The increased expression of these mucin genes were significantly suppressed by 100, 10, and 1 ng/mL of Omnaris. ConclusionZZOmnaris significantly suppressed the production of chemical mediators and mucin gene expression, which indicated that Omnaris is effective in improving and treating inflammatory diseases in the nasal cavity. Korean J Otorhinolaryngol-Head Neck Surg 2014;57(9):603-9