The Effect of oestradiol benzoate on the duration of oestrus and release of LH in ovariectomized Galway ewe lambs and adult ewes

Abstract

The oestrous and LH responses by ovariectomized adult ewes ( N=23) and 8-month-old ewe lambs ( N=24) to i.m. injection of 10, 25, 62.5 or 156.25 μg oestradiol benzoate (ODB) were compared. The animals were primed by six daily injections of progesterone and ODB was administered 48 h after the last progesterone injection. The interval between ODB injection and onset of oestrus declined linearly ( P<0.01) as the dose of ODB increased and was similar for the two age groups. The mean (±SEM) intervals to oestrus for levels of 10, 25, 62.5 and 156.25 μg ODB were 22.9±1.90, 18.0±1.33, 14.5±1.26 and 13.5±1.32 h, respectively. The duration of oestrus, determined by checking with Finnish Landrace rams at 3-h intervals, increased linearly ( P<0.01) as the dose of ODB was raised and was significantly longer for ewe lambs (63.1±2.95 h) than for adult ewes (50.4±3.52 h). The overall mean (±SEM) durations of oestrus for levels of 10,25, 62.5 and 156.25 μg ODB were 16.9±5.91, 37.0±4.13, 75.2±3.94 and 97.8±4.13 h, respectively. A “pre-ovulatory” -type LH surge was observed in 32 of the 47 animals studied. The interval between injection of ODB and the beginning of the LH release declined as the dose of ODB increased ( P<0.01) and was shorter ( P<0.01) for ewe lambs (19.8±0.74 h) than for adult ewes (23.2±0.90 h). There was no evidence for an effect of either ewe age or dose of ODB on the maximum LH concentration observed, duration of LH discharge or total quantity of LH released. The sensitivity of the two age groups to the negative feedback effects of ODB on LH secretion was similar.