The effect of normobaric hyperoxia on the spatial learning and memory of APP/PS1 double transgenic mice

Abstract

Objective To investigate whether normobaric hyperoxia exert neuroproteetive effect on APP/ PS1 double transgenic AD mouse model. Methods 20 APP/PS1 transgenic mice were randomly divided into 2 groups(A, B). Mice in group A were treated with 40% oxygen for 8 h per day, and lasted 8 weeks. Mice in group B were treated with normal air, as control. ELISA assay as well as behavioral test were used in the present study. Results Compared with normoxia-treated control, hyperoxia-treated mice had a significant lesser (P 0.05 ). EL1SA showed that Aβ40(783.64±97.21)pg/ml and Aβ42(175.30 ± 17.09) pg/ml were significantly decreased in hyperoxia-treated mice, compared with control[Aβ40 (1251.59 ± 42.29 ) pg/ml and Aβ42 (286.83 ± 12.96) pg/ml] (P< 0.01 ). Conclusion Treatment with hyperoxia significantly decreases Aβ deposition, and remarkably improves the capability of spatial learning and memory of APP/PS1 transgenic mice. Hyperoxia may exert its neuroprotective effect through decreasing Aβ generation or enhancing clearance of Aβ by endothelial cells. Key words: Normobaric hyperoxia; Alzheimer's disease; Learning and memory; Aβ amyloid