The effect of nonsteroidal and steroid anti-inflammatory drugs on platelet activation IN VITRO

Abstract

The article presents the results of a study on the effect of steroid and nonsteroidal anti-inflammatory drugs on platelets.The object of the study was plasma obtained  from  rat  blood  (n=10).  Blood  sampling (2.0 ml from each animal) was carried out  from  the  tail  vein  into  test  tubes  with sodium citrate. Cell elements were counted directly in blood and in platelet plasma using a MEK-6550 hematological analyzer.The  ability  to  influence  platelet  activation was determined in drugs such as dexamethasone,  ketarol  and  miloxicam,  which were injected into test tubes before plasma reactivation. It  was  found  that  the  drugs  of  these groups affect the metabolism of these cells and inhibit their ability to cause clot contraction.  It  was  found  that  the  strength  of  the negative  effect  on  clot  contraction  in  the steroid drug dexamethasone, which through intermediaries  suppresses  the  activity  of phospholipase-A2 and the nonsteroidal drug ketarol,  which  is  the  predominant  inhibitor of cyclooxygenase of the first type, are similar.  It  was  also  found  out  that  the  drug meloxicam, which is a predominant inhibitor of cyclooxygenase of the second type, causes only a slight decrease in platelet clot contraction. The results obtained in the experiment indicate  a  direct  positive  relationship  between the degree of clot contraction and the amount  of  thromboxane-A2,  which  is  synthesized and released by activated platelets. It  is  known  that  the  ability  of  platelets  to accelerate and enhance regenerative processes is also directly related to their activation. Based  on  this,  it  is  suggested  that  patients who have been  prescribed  plateletenriched plasma  therapy  should  not  use  glucocorticoids and preparations with a high degree of selectivity  to  cyclooxygenase  of  the  first type as anti-inflammatory drugs.