Abstract

In a greenhouse pot experiment with kohlrabi, variety Luna, we explored the joint effect of N (0.6 g N per pot = 6 kg of soil) and S in the soil (25-35-45 mg kg-1 of S) on yields, on N, S and NO3- content in tubers and leaves, and on alterations in the amino acids concentration in the tubers. S fertilisation had no effect on tuber yields. The ranges of N content in tubers and leaves were narrow (between 1.42-1.48 % N and 1.21-1.35 % N, respectively) and the effect of S fertilisation was insignificant. S concentration in the tubers ranged between 0.59 and 0.64 % S. S fertilisation had a more pronounced effect on the S concentration in leaf tissues where it increased from 0.50 to 0.58 or to 0.76 % S under the applied dose. The NO3- content was higher in tubers than in leaves. Increasing the S level in the soil significantly reduced NO3- concentrations in the tubers by 42.2-53.6 % and in the leaves by 8.8-21.7 %. Increasing the S content in the soil reduced the concentration of cysteine + methionine by 16-28 %. The values of valine, tyrosine, aspartic acid and serine were constant. In the S0, S1, and S2 treatments the levels of threonine, isoleucine, leucine, arginine, the sum of essential amino acids and alanine decreased from 37 to 9 %. The histidine concentration increased with increasing S fertilisation. S fertilisation of kohlrabi can be recommended to stabilize the yield and reduce the undesirable NO3- contained in the parts used for consumption.

Highlights

  • An acute deficiency of sulphur (S) in the soil has been observed in Europe since the 1980ies

  • The reduced soil content of available S is the result of reduced applications of mineral and organic fertilisers and fungicides (Hlušek et al, 2003)

  • Colorimetry was used to determine the content of available P in the extract and atomic absorption spectrophotometry (AAS) to determine available K, Mg and Ca

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Summary

Introduction

An acute deficiency of sulphur (S) in the soil has been observed in Europe since the 1980ies. In the Czech Republic this phenomenon was intensified after 1990. Vegetables requiring a higher supply of S began to respond very sensitively. One of the most demanding vegetables is kohlrabi, which absorbs 1.5 kg S per ton yield. It is the most S–dependent of all brassicas. The typical visual S deficiency symptom in kohlrabi is inhibited leaf formation and a light colour of the youngest leaves. The reduced soil content of available S is the result of reduced applications of mineral and organic fertilisers and fungicides (Hlušek et al, 2003)

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