Abstract
AbstractThis research was conducted using beef extracted from Kupang (se’i meat), Indonesia. Se’i meat is a locally found food where the preferred mode of preparation is smoking the beef with the preservation using nitrites. Nitrite can cause health-related problems such as cancer. This research was carried out using a true experimental method with a complete randomized design with the aim of analyzing the effect of meat administration on the expression of wild-type p53 protein in colon cells of Balb/c mice as an indicator of carcinogenesis. The measurement of p53 is to observe the increase in the-over-capacity of p53 expression in the colon cell as a result of decrease in wild-type protein p53. This research provides scientific information about the effect of giving se’i meat on the expression of wild-type p53 in cells of Balb/c mice as an indicator of carcinogenesis. A total of 36 male mice of Balb/c strain weighing 23.8 g were divided into four groups classified as samples (P1, P2 and P3) and control (K), which were taken from modern and home industries in the city of Kupang. The results showed that consumption of nitrite-preserved beef se’i (traditional smoked meat) increased the p53 protein expression in colon cells of Balb/c strain male mice, and the least significant difference test also showed that there were differences in wild-type p53 protein expression among the four groups: P1 (mice that have been given the standard food, drinking water and se’i meat that contains no nitrite) has an average of 142 expressions, which is higher than that of P3 (mice that have been given the standard food, drinking water and se’i meat containing nitrite which come from the home industry) which has an average of 106.55 expressions and is higher than that of K (mice that have been given the standard food and drinking water) which has the total average of expression of about 78.11 expressions. The benefit of this research is to gain the scientific information about the effect of giving smoked meat on the expression of wild-type p53 in colon cells of Balb/c mice as a carcinogenic indicator.
Highlights
Promoting healthy living has been a challenge for a global society, and the need to increase nutritional consumption plays an important role in supporting lifestyles [1,2,3]
We report about the consumption effect of se'í beef meat that contains nitrite compounds on the effect of wild-type p53 expression protein produces in mice colon cells
Nitrite is a salt of weak acids and strong bases, in the form of crystals that are pale yellow and soluble in water
Summary
Promoting healthy living has been a challenge for a global society, and the need to increase nutritional consumption plays an important role in supporting lifestyles [1,2,3]. Meat is a nutritional food that is almost inseparable from human life; the quality and availability in the community are often not guaranteed. Meat contains proteins that are one of the important food substances for the body, such that they have functions such as cell growth, replacement of damaged cells and as fuel in the human body. Due to its nutritional value, se’i meat (local name) is one of the processed smoked beef that has a high protein content of 30–32%, a fat content ranging from 0.8 to 0.92% and a high water content of 63%, causing it to be contaminated by microbes which results in a very short shelf life of approximately 3 days [10]
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