The Effect of Nimodipine on Opioid Antagonist-Induced Upregulation and Supersensitivity

Abstract

Listen

Translate article

Regulation of calcium flux has been suggested to play a role in acute and chronic effects of opioids. Previous studies have shown calcium channel blockers can inhibit opioid agonist-induced downregulation of μ-opioid receptors and may reduce the magnitude of tolerance. In the present study, we determined if calcium channel blockade would affect increases in opioid receptor density and functional supersensitivity produced by chronic opioid antagonist treatment in the mouse. Mice were implanted subcutaneously with a 15-mg naltrexone (NTX) or placebo pellet. Mice also were implanted with an osmotic minipump that infused nimodipine (100 μg/kg/day) or a second placebo pellet. This protocol yielded four groups: nimodipine–NTX; nimodipine–placebo; placebo–NTX; placebo–placebo. On the seventh day, pumps and pellets were removed. Twenty-four hours later, a morphine dose–response study was conducted (tail flick); or mice were sacrificed and saturation binding studies ([ 3H]DAMGO) were performed in whole brain. NTX treatment significantly increased the analgesic potency of morphine by ∼60%. Nimodipine increased the potency of morphine by ∼50%. For mice treated with both nimodipine and NTX, there was an additive effect on morphine potency (∼120% increase). In binding studies, NTX increased the density of μ-opioid receptors similarly (∼60–70%) in the presence and absence of nimodipine treatment, with no change in affinity. No effect of chronic nimodipine alone on μ-opioid receptor binding was observed. These data indicate that NTX-induced upregulation and supersensitivity are independent of calcium channel blockade by nimodipine. These results contrast with those from tolerance and downregulation studies, and confirm suggestions that different substrates mediate chronic opioid agonist and antagonist-induced effects in vivo. Finally, in a separate study, morphine potency was unaffected by acute nimodiopine (100 μg/kg; SC), suggesting that prolonged exposure to this calcium channel blocker is required to increase morphine potency.