Abstract
The biological activity of biopolymer-based selenium nanocomposite with a high selenium content of 6.4% was investigated. It was shown earlier that nanocomposites of selenium and arabinogalactan (NC Se/AG, 1.23% and 3.4% Se) have a bactericidal and bacteriostatic effect on the pathogenic bacterium Clavibacter michiganensis subsp. sepedonicus (Cms) and do not adversely affect the vitality of potato plants in vitro. In this work, it was shown that the nanocomposite under scrutiny has a bacteriostatic effect, inhibits bacterial growth by 20% compared with the check variant and reduces the ability of Cms to form biofilms, which contribute to their high resistance to external factors. In the experiments conducted on plants, it was shown, there was not negative effect of NC Se/AG on biometric parameters, furthermore, this NC reduces the negative effect of potato infection with Cms. The nanocomposite reduced the activity of peroxidase and the content of reactive oxygen species in potato tissues. It was established that selenium does not accumulate in potato plants after the treatment with NC Se/AG. The results obtained allow us to consider NC Se/AG (6.4% Se) as an agent for healing cultivated plants from pathogenic bacteria.
Highlights
Работа выполнена при частичной финансовой поддержке гранта Президента Российской Федерации для молодых ученых NoМК-1220.2019.11, гранта РФФИ и Правительства Иркутской области
It was shown that the nanocomposite under scrutiny has a bacteriostatic effect, inhibits bacterial growth by 20% compared with the check variant and reduces the ability of Clavibacter michiganensis subsp. sepedonicus (Cms) to form biofilms, which contribute to their high resistance to external factors
It was shown earlier that nanocomposites of selenium and arabinogalactan (NC Se/AG, 1.23% and 3.4% Se) have a bactericidal and bacteriostatic effect on the pathogenic bacterium Clavibacter michiganensis subsp
Summary
Для исследования бактериостатической активности биокомпозитов селена в отношении кольцевой гнили картофеля жидкую культуру Cms выращивали в темноте при 26 °С на качалке (80 об./мин) в колбах, содержащих питательную среду GPY, рН 7.2. Влияние нанокомпозита селена на биопленкообразование Cms исследовали с применением планшетного метода [16]. Для проведения эксперимента в ростовую среду картофеля вно-. Для выявления областей продукции активных форм кислорода (АФК) растения заражали Cms, выдерживали 2 сут, затем обрабатывали нанокомпозитом, спустя 1 ч кокультивирования осуществляли подготовку проб для анализа. Полученные препараты заключали в ProLong Gold antifade reagent (Thermo Fisher Scientific Inc., USA) и исследовали в LSM 710 (Zeiss, Germany). Растения заражали Cms, через 2 сут, когда оно полностью колонизировано патогеном, его обрабатывали нанокомпозитом. Спустя 2 сут кокультивирования в факторостатных условиях осуществляли подготовку проб для анализа. Полученные данные подвергали статистической обработке с использованием пакета программ MS Excel
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