Abstract
Along with the increasing quantity of agriculture in Indonesia, should be followed by improving the quality of agriculture. Generally one of the indicators or parameters commonly used to indicate the quality of agriculture is the number of pests that damage plants in certain areas. The control of disturbing organisms on the plant can be minimized by the bio-insecticide production of Carica papaya which is tapped and extracted with ultrasonic-assisted using a mixed NADES solvent from Cholin chloride (ChCl) and Oxalic acid (Ox) with varying sonification time for 10, 20, 30 minutes and solvent ratio of 1: 2 and 2: 1. Bio-insecticide was produced by conducting qualitative enzyme activity of with lowry method and quantitatively using UV / Vis spectrophotometer aid with 750 nm wavelength and efficacy test. The best extraction result was obtained at NADES solvent with 1: 2 ratio with extraction time for 30 min with extraction time for 30 minutes yielding protein concentration equal to 7,23 ppm and tyrosine concentration equal to 7,9 ppm. Bio-insecticide samples effectively kill grayak caterpillars with 100% mortality within 5-7 days.
Highlights
Indonesia is an area with abundant natural resources, making Indonesia known as an agrarian country
The discussion includes the amount of protein concentration, the amount of cysteine protease enzyme with the activity of papain enzyme extract for variation of the solvent ratio and the extraction time of the extract produced
Compared with a ratio of 2: 1 or 2 which has a protein concentration at 10, 20 and 30 minutes time variations of 5.24, 5, 78, 6.11 ppm. The acquisition of this protein concentration when viewed qualitatively as to why ethanol is higher than that of NADES solvent used is when the yield of the extract using ethanol is reacted with the biuret and the Folin- Ciocalteu reagent produces a less intense blue color which means it has a fairly high protein content
Summary
Indonesia is an area with abundant natural resources, making Indonesia known as an agrarian country. Because it has a very potential area to develop businesses in the agricultural sector. Some examples of natural resources from the agricultural sector are like soybeans, taro, broccoli and others. This natural resource is widely used for consumption of raw materials for food and feed industry. In an effort to increase the production of natural resources need to consider how optimal production is produced from agricultural land that is protected from pests. When the papaya plant is injured, the sap will come out and the enzymes will become active. Protease cysteine enzymes have the potential to be developed as bio-insecticide active ingredients. The bio-insecticide production was first Carica papaya tapped and extracted with ultrasonic waves using NADES solvent. Bio-insecticides are produced by performing quantitative tests of proteins by lowry and efficacy test
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