The effect of multiband sequences on statistical outcome measures in functional magnetic resonance imaging using a gustatory stimulus

Abstract

Recent technical developments have led to the invention of multiband functional magnetic resonance imaging (fMRI) sequences that allow for faster sampling rates. However, some studies have highlighted problems with these sequences, leading to a decreased temporal signal-to-noise ratio (tSNR). In addition, this temporal noise may interfere with detecting reward-related responses in mesolimbic regions. The blood-oxygen-level-dependent signal utilized in the majority of fMRI measurements is relatively slow. Furthermore, the cerebral response to gustatory stimuli would also be relatively slow. Therefore, given the temporal noise issues with multiband sequences, it is unclear whether multiband sequences are necessary for fMRI studies using gustatory stimuli. We thus conducted an fMRI experiment using a gustatory stimulus to investigate the effects of multiband sequences and increased sampling rates on statistical outcome measures. A single-band sequence with a repetition time (TR) of 2 s of phantom fMRI data and gustatory fMRI data from the gustatory regions exhibited the highest tSNR, although the tSNR of this sequence of gustatory fMRI was not statistically different from tSNR of multiband sequences with a TR of 2 s in any of the selected region of interests. Conventional general linear model analysis of fMRI showed that single-band sequences are more advantageous than multiband sequences for detecting brain responses to gustatory stimuli in the primary gustatory cortex. In addition, a Bayesian data comparison showed that data derived from a single-band sequence with a TR of 2 s was optimal for inferring neuronal connectivity in gustatory processing. Therefore, a conventional single-band sequence with a TR of 2 s is more appropriate for fMRI with gustatory stimuli. Image acquisition sequences should be selected aligned with the study objectives and target brain regions.