The effect of Moringa oleifera (Lam) leaves ethanol extracts as anti-inflammatory and anti-fibrotic through TNF-α and p38-MAPK expression: In vivo model of liver fibrosis approach

Abstract

Recently, no anti-fibrotic agents work effectively to suppress fibrosis process because of its complex pathophysiology. Moringa oleifera (MO) has been shown to function as anti-inflammatory and anti-fibrotic agent through inhibition of NFĸB cascade pathway. This study was conducted to prove the anti-inflammatory and anti-fibrotic effects of MO for inhibit liver fibrosis process through an expression of TNF-α and p38-MAPK. Male Rattus norvegicus strain Wistar rat 3 months old separated randomly was conducted in this study. The liver fibrosis rat model was injected CCl4 10% 1 - 2 cc/kg BW twice a week during 14 weeks. The treatment groups which received MO ethanol extract were divided into three doses (150, 300, and 600 mg/kg BW). MO ethanol extract was injected shortly after CCl4 injection through oral gavage. Samples were analyzed for TNF-α and p38-MAPK expression using immunohistochemistry. Expression of TNF-α and p38-MAPK are decrease in all treatment groups using MO (150, 300, 600 mg/kg BW) compared to control group significantly (p<0.001). The result shows MO has anti-inflammatory and anti-fibrotic effects as expected. Moringa oleifera was proven to be able to decrease TNF-α and p38-MAPK in treatment groups compared to rat models. It could be a potential herbal as anti-inflammatory and anti-fibrotic to inhibit fibrosis process.