The Effect of Moringa Leaf Extract on Malondialdehyde Levels in Male Wistar Rats

Abstract

Excessive physical activity will result in body fatigue. This causes oxidative stress that occurs due to an imbalance between oxidants and antioxidants which can then cause damage characterized by MDA. Moringa leaf extract is an antioxidant from outside the body that is useful in reducing fatigue. This study aimed to decrease MDA levels in male Wistar rats by Moringa leaf extract. We gave swimming exercises to tire the mice. It will increase the levels of MDA in their blood. Fatigue can be characterized by a variety of biological signs. The difference in our research lies in the biological marker used as an indicator of fatigue, namely MDA levels. The sample used in this study consisted of 4 groups to be given treatment, with each group consisting of 5 rats. MDA levels were tested using the competitive ELISA (enzyme-linked immunosorbent assay) method. One-way ANOVA test was used to find out where the difference was, with 95% correct confidence for this test, and if p < 0.05, a significant difference was obtained. The lowest mean MDA levels were in the treatment of Moringa extract at 300 mg/kg in the pre-test, the first week, and the second week, 1.82 ± 0.20, 1.24 ± 0.03, and 0.86 ± 0.12, respectively. The results of the one-way ANOVA test in this study showed differences in each treatment group with p= 0.000. The results of the examination of MDA levels at each treatment time showed a difference between before the treatment, the first week, and the second week.