Abstract
The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P 0 ), 10% (P 1 ), 20% (P 2 ), and 30% (P 3 ). The result showed that the percentage of sperms abnormality in P 0 , P 1 , P 2 , and P 3 were respectively 8.67%, 6.67%, 5.67%, and 10%. P 2 was the lowest percentage, while P 3 was the highest percentage of sperms abnormality. In addition, this research found twelve kinds of sperms abnormality divided into categories: major abnormality included the broke tail, absent head, detached head, dag defect, tapered head, proximal droplet, round head and abaxial; and minor abnormality included the coiled tail, shoe hook tail, bent tail, and distal droplet. It has been concluded that the allotment of 20% Morinda citrifolia L. extracts in Tris-egg yolk medium able to maintain the sperm morphology at -80°C storage for 24 hours. Keywords: goat, Morinda citrifolia L., post-thawing, spermatozoa.
Highlights
Goat, cattle and its production are very potential to be developed for filling domestic and export market
The effect of the freezing process toward the increment of free radicals has been confirmed in 2001 using cow’s spermatozoa; it showed the increasing of ROS during sperm production until post-thawing process [11]
Awada and the relatives presented that the effect of free radicals induced the rise of hydrogen peroxidation, lipid peroxidation, and reduce motility spermatozoa of Boer goat [12]
Summary
Cattle and its production are very potential to be developed for filling domestic and export market. Increasing of goat population through reproduction efficiency with biology reproduction technology providing in Livestock Research Center or other institution is one of the methods to fill goat consumption needs [2]. Thawing causes drastic temperature alteration, it increases metabolism, augmenting the concentrate of free radicals [9]. The effect of the freezing process toward the increment of free radicals has been confirmed in 2001 using cow’s spermatozoa; it showed the increasing of ROS during sperm production until post-thawing process [11]. Awada and the relatives presented that the effect of free radicals induced the rise of hydrogen peroxidation, lipid peroxidation, and reduce motility spermatozoa of Boer goat [12]
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