Abstract
This study was conducted to investigate the effect of MSG treatment for short and long term on the semen quality of adult male rats. Twelve male adult Wistar rats with 200-300g of body weight (BW) and 3-4 month of age were used in this study. The animals were divided randomly into 3 groups. M0 was used as a control, M1 and M2 were given with MSG 4mg/gBW for 15 and 45 days respectively. The experimental animals were sacrificed on the days 16 th (to M1 group) and 46 th (to M0 and M2 groups). The epididymis was isolated and semen quality (motility, viability, concentration, and abnormality of sperm) was evaluated. The results showed motility and concentration of M1 and M2 were not significantly decreased compared to M0. MSG treatment also significantly reduced viability and increased abnormality of sperm. Analysis of sperm abnormality character shows that the use of long-term MSG caused a formation of the primary abnormality (round and double head sperm) and increased the secondary abnormality (bent neck, curve tail, coiled tail, headless, and tailless) compared to control. Conclusion, semen quality decreases with consumed MSG for the long term. For this reason, reconsidering the use of MSG as an enhancer for the teste of food is very important. Keywords: epididymis, Monosodium L-Glutamate, semen quality.
Highlights
INTRODUCTION* Monosodium L-Glutamate (MSG) is a white crystal-like substance that contains 78% of glutamic acid, 22% of sodium and water [1]
Treatment of MSG at 4mg.g body weight (BW)-1 in rats for 14 and 28 days induced alteration of sperm function in testes include significantly decrease of conversion of spermatogonia to primary spermatocytes and increase the number of inactive spermatogonia compared to controls [8]
The treatment of MSG at longer term for 45 days caused a very significantly reduced of the sperm viability from adult male rats
Summary
200-350g body weight (BW) were obtained from the Animal Unit Laboratory of Gajahmada University, Yogyakarta, Indonesia. The motility, viability, abnormality, and concentration of sperm were examined by a light microscope at 100 and 400x magnifications. Sperm Motility (SM) Total of 10μL semen were added to object glass. Sperm Viability (SV) and Abnormality (SA) Total of 10μL liquid semen was added to object glass and was stained with 1% eosin/5%. Percentage of sperm viability and abnormality was examined by a light microscope at 400x magnification in 3 microscope fields (or up to 200 cells). This is the formulation [12,14]: Description: SV = sperm viability SA = sperm abnormality. Statistical Analysis The data of control and experimental group were presented as mean values and Standart Error (SE). Result of one way ANOVA analysis is significant difference at P
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