The effect of modern hepatotoxicants on the elimination of caffeine

Abstract

Hepatotoxicants are a variety of chemical substances that can have organ-specific damaging effects, which may be the result of their direct effects on both hepatocytes and other structural units of the liver. The purpose of this study is to evaluate the effect of modern hepatotoxicants on caffeine elimination. The studies were carried out in the vivarium of the Department of Pharmacology and Toxicology of the Federal State Budgetary Educational Institution of Higher Education of St. Petersburg State University of Medicine. Industrial substances of different chemical groups have been used to induce hepatotoxic conditions: phthalic anhydride, ethylenediamine and chloroform. All of the above toxicants were given in dosages for the immediate (within 24 hours) induction of hepatotoxic conditions. One day after the completion of the simulation, the levels of caffeine in the blood serum of the experimental animals were determined in full accordance with the previous series of experiments, using a dosage of 100 mg/kg, since with this dosage regimen we identified the most stable pharmacokinetic profiles of caffeine. Mathematical and statistical processing of the obtained data was carried out using the STATISTICA 6.1 program. The arithmetic mean (M) and its mean error (m) were calculated; the significance of the difference (p) was not calculated using the Student's test due to the exploratory nature of the study and the lack of comparison groups. Based on the conducted research, we can conclude that each hepatotoxicant has individual characteristic characteristics, which allows us to recommend a caffeine test for different types of hepatotoxicity, incl. unexpressed. That is, in this way it is possible to diagnose hepatotoxic conditions even in a latent form, which can be used as a method for assessing the toxicity of a drug at the stage of preclinical development.