The effect of moderate exercise on the elevation of Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene.

Anis Irmawati,Sidarningsih Sidarningsih,Nadira Jasmin

doi:10.14202/vetworld.2018.177-180

Anis Irmawati, Sidarningsih Sidarningsih + Show 1 more

Open Access

https://doi.org/10.14202/vetworld.2018.177-180

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Journal: Veterinary world	Publication Date: Feb 1, 2018
Citations: 4	License type: CC BY 4.0

Affiliation: Airlangga University

Abstract

Aim:The aim of this study was to analyze the effect of moderate exercise on the elevation of Bax/Bcl-2 ratio.Materials and Methods:Eighteen Mus musculus strain Swiss Webster (Balb/c) were divided into three groups (n=6). K1 and K2 had contact with water 3 times/week for 12 weeks, while the members of the K3 group swam 3 times/week for 12 weeks while carrying load weighed 3% of their body weight. After 5 weeks, they were induced with 0.04 ml oleum olivarum (K1), 0.08 mg benzopyrene/0.04 ml oleum olivarum (K2, K3) 3 times/week for 4 weeks. Immunohistochemistry assays were used to determine the ratio of Bax/Bcl-2 expression. The results were analyzed using an independent t-test.Result:The Bax/Bcl-2 ratio increased significantly in K3 compared to K2 (p=0.00).Conclusion:Moderate exercise could increase the Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene.

Highlights

Cancer is a leading cause of death worldwide
Moderate exercise could increase the Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene
This study aims to analyze the effects of physical exercise as a method of preventing cancer by investigating the influence of moderate-intensity physical exercise in the elevation of Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene

Summary

Introduction

Cancer is a leading cause of death worldwide. According to Riset Kesehatan Dasar (riskesdas), in 2013 the prevalence of cancer in the population of all ages in Indonesia amounted to 1.4%. The percentage of oral cavity and lip cancer cases worldwide in 2012 represented 4.0% of new cases with 1.9% mortality rate [1]. The incident rate of smoking, the biggest risk factor for oral cavity cancer, is still high in Indonesia. Benzopyrene is one PAH compound that has been widely studied. It is metabolized by oxidative enzymes, especially cytochrome p450 [4]. After going through several stages of metabolism, benzopyrene will produce carcinogenic metabolites, e.g. Benzopyrene 7,8 diol-9,10-epoxide (BPDE) which cause mutant cells formation [5]

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