Abstract
Microwave irradiation may alternate reaction specifity in multipath reactions. In this experiment, transglycosylation of stevioside with starches or α/β-cyclodextrin (CD) reached reaction equilibrium only in 3min under low power microwave irradiation, when 10U/g stevioside of a CGTase was applied. Microwave irradiation does not dramatically affect the transglycosylation pathway of the starches with stevioside, but it remarkably affects the transglycosylation pathway of α/β-CD onto stevioside. Unlike the case under conventional heating, the transformation between α-CD and β-CD was dramatically declined by microwave. With α/β-cyclodextrins as glycosyl donor, highly grafted products were diminished under microwave irradiation. Gelatinized starch produced much more cyclodextrins than hydrolyzed starch did, which made higher stevioside conversion with gelatinized starch.
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