Abstract
Methylglyoxal bis(guanylhydrazone), an inhibitor of S-adenosyl-L-methionine decarboxylase, inhibits partially the yield of infectious, progeny virus in vaccinia-infected HeLa cells. This effect is reversed by the simultaneous addition of spermidine showing that this polyamine is required for optimal virus growth. Although DNA, RNA and protein synthesis are unaffected, the formation of DNA-containing, cytoplasmic inclusions characteristic of vaccinia infection occurred at a reduced frequency compared with non-inhibited controls. Thus, the association of virus DNA with cytoplasmic inclusions is decreased in the absence of spermidine synthesis. The nature of residual virus replication in the presence of the inhibitor is discussed.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
