The effect of mesenchymal stem cells from the adipose tissue and bone marrow of goats on the quality of their sperm cells

Abstract

The purpose of this research is to study the influence of mesenchymal stem cells from adipose tissue and bone marrow of goats on the quality parameters of their sperm. A complex evaluation of sperm quality (volume, concentration, morphology, motility) of Saanen and Czech goats (n=10) at the age of 1-2.5 years was carried out according to generally accepted methods and protocols. Adipose tissue (AT) and bone marrow (BM) were obtained after the slaughter of goats (n=6) on a peasant farm in the Volkhov district of the Leningrad region. After processing the obtained samples, mesenchymal stem cells obtained from adipose tissue and bone marrow of goats were isolated, accumulated and cryopreserved. Three groups of samples of 10 each were formed: control (spermatozoa 7x1010 cells/ml + PBS buffer 100 μl, pH – 7.4), first experimental (spermatozoa 7x1010 cells/ml + MSCs 2x1010 cells/ml, pH – 7.2), the second was experimental (sperm 7x1010 cells/ml + BM MSCs 2x108 cells/ml, pH – 7.2) and a four-stage assessment of sperm quality was carried out: after incubation of sperm with mesenchymal stem cells at a temperature of 38℃ for 0, 1, 2 and 3 hours. Based on the results obtained, the working protocol for the use of mesenchymal stem cells from adipose tissue and bone marrow for the sperm of breeding goats is the concentration of MSCs - 2x108 cells/ml and the concentration of goat spermatozoa - 7x1010 cells/ml. The studies conducted have established the positive effect of mesenchymal stem cells from the adipose tissue and bone marrow of goats on the quality indicators of their sperm. At the same time, spermatozoa remain viable during three-hour incubation with a progression of movements equal to 51.5±8.46 - 51.8±5.2% and a number of morphologically normal sperm equal to 50.40±2.21 - 51.10±3, 14% (3 hours after incubation, MSCs from adipose tissue and bone marrow, respectively).