Abstract
Mercaptoethanol (EtSH) was shown to enhance the solubility of the 39.5 kDa outer membrane (OM) protein of elementary bodies of chlamydia trachomatis in detergents. In the presence of mild detergents and EtSH almost selective solubilization of the 39.5 kDa protein and chlamydial LPS was obtained. This solution was further applied to gel filtration in the presence of SDS to purify the 39.5 kDa protein free of LPS.
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