Abstract
An important factor in determining epithelial cell barrier integrity in an invitro system is the support membrane on which cells are cultured. In this work a custom airway barrier on chip platform was used to investigate the impact of various support membranes on bronchial epithelial cell growth and barrier formation. The membranes had different pore sizes (0.4 μm or 3 μm), densities (1 × 108, 8 × 105, 1.6 × 106 pores/cm2), thicknesses (9 μm, 12 μm, 20 μm) and chemical treatments (polyvinylpyrrolidone or oxidative). Trans Epithelial Electrical Resistance (TER) data for the various cell constructs was compared with cells grown on commercial Transwells®. The results demonstrate differences in barrier integrity and structure, with cells grown under flow having reduced barrier integrity (lower TER) compared to the same cells grown without flow. Bronchial epithelial cells grown in the dynamic (with flow) barrier on a chip platform consistently grew on 0.4 μm pore membranes with higher TERs, compared to 3 μm pore membranes with thinner cell layers due to cell migration where cells translocated to the basolateral region.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
