Abstract
This study examined the metabolism of free radicals in hepatic mitochondria of goats induced by lipopolysaccharide (LPS), and investigated the effects of melatonin (MT). Forty-eight healthy goats () were randomly selected and divided into four groups: saline control, LPS, MT+LPS and MT. The goats within each group were3 sacrificed either 3 or 6 h after treatment and the livers removed to isolate mitochondria. The respiration control ratio (RCR), the ADP:O ratio, the oxidative phosphorylation ratio (OPR), the concentration of and the activities of Complex I-IV were determined. The mitochondrial membrane potential () was analyzed by flow cytometry. The results showed that RCR, O/P and OPR of the LPS group decreased (p in Complex III increased (p. Interestingly, it is revealed that, in the MT+LPS group, the generation of increased firstly in 3 h, and then significantly (p were obviously improved compared to the control group. Conclusion: melatonin prevents damage caused by LPS on hepatic mitochondria of goats.
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