The Effect of Mast Cells Stimulated by Toll-Like Receptors on Keratinocytes

Abstract

Abstract The mast cells, as located in the skin’s dermal layer, are important innate immune cells that mediate inflammation as a defense response against outer pathogens. Mast cells are activated by innate immune receptors such as Toll-like receptors receptrs (TLRs) and interact with a variety of cells including keratinocytes. This study confirmed the effect of TLRs-stimulated mast cells on the migration of keratinocytes residing in the epidermis. The HaCaT cells, a human keratinocyte cell line, was treated with the concentrated conditioned medium (CM) of the HMC-1, a human mast cell line. After that, the migratory capacity via scratch and transwell assays. In addition, we tried to confirm whether TLRs activations affect these effects of mast cells on keratinocyte migration. Thus the same experiments were carried out using the CM of HMC-1 obtained under various stimulation by TLRs agonist. As a result, HMG-1 CM promoted the migratory ability of HaCaT cells in scratch assay and transwell assay. Those effects were further increased when HMC-1 was exposed to various TLRs stimulation. This work was supported by a grant of the National Research Foundation of Korea (NRF) grant funded by the Korea Government (MSIT) (NRF-2021R1A2C1012551).