The effect of Lycium barbarum on reproductive system and the expression of CRISP-1 protein in experimentally diabetic male rats

Abstract

Aim: The main purpose of this study is to investigate the effects of Lycium barbarum polysaccharides (LBP) on the male reproductive system and Crisp-1 protein expression in experimentally diabetic Wistar Albino male rats.
 Material and Method: In this study Wistar Albino male rats (3-4 months old) were randomly selected and divided into four groups; Control, LBP control (only LBP), Diabetic and Treatment (diabetic + LBP) group. For the experimental diabetes model, a single dose of 55 mg/kg STZ was injected intraperitoneally. In the treatment group, the diabetic rats were administered with 200 mg/kg of LBP by gastric gavage for 15 consecutive days. 
 Results: In histological examinations, increased intertubular connective tissue, congestion, vacuolization and edema were observed in testicular tissues of the diabetic group. The histopathological changes were improved after LBP treatment. Also, the number of total sperm count and sperm motility were significantly increased in the treatment group. Our biochemical analysis results showed that the serum testosterone level were significantly increased and serum MDA level were significantly decreased after treatment with LBP. Compared to the diabetic group, the apoptotic cells were decreased in the treatment group. Crisp-1 protein expression was increased in the treatment group, Crisp-1 possitive vesicle-like structures and apical blebs were also examined in the epididymal tissues.
 Conclusion: It is concluded that Lycium barbarum polysaccharides have a therapeutic effect on the male reproductive damages of diabetes and also enhances Crisp-1 protein expression in the epididymis.