Abstract
This study investigates the effect of Dexamethasone (Dex) treatment on blood and skeletal muscle metabolites level and skeletal muscle activity of enzymes related to energy metabolism after long-duration swimming. To evaluate whether Dex treatment, swimming, and combining these factors act on analyzed data, rats were randomly divided into four groups: saline treatment non-exercise and exercise and Dex treatment non-exercised and exercised. Animals in both exercised groups underwent long-lasting swimming. The concentration of lipids metabolites, glucose, and lactate were measured in skeletal muscles and blood according to standard colorimetric and fluorimetric methods. Also, activities of enzymes related to aerobic and anaerobic metabolism were measured in skeletal muscles. The results indicated that Dex treatment induced body mass loss and increased lipid metabolites in the rats’ blood but did not alter these changes in skeletal muscles. Interestingly, prolonged swimming applied after 9 days of Dex treatment significantly intensified changes induced by Dex; however, there was no difference in skeletal muscle enzymatic activities. This study shows for the first time the cumulative effect of exercise and Dex on selected elements of lipid metabolism, which seems to be essential for the patient’s health due to the common use of glucocorticoids like Dex.
Highlights
IntroductionOther adverse events of Dex administration are skeletal muscle atrophy and accelerated weight loss [10,11]
Neither nine days of Dex treatment and three hours of swimming nor a combination of these conditions resulted in any changes in activities of enzymes related to aerobic (citrate synthase (CS) and cytochrome c oxidase (COX)) and anaerobic (lactate dehydrogenase (LDH)) metabolism in both SOL and extensor digitorum longus (EDL) skeletal muscles (Table 2) as well as the level of succinate dehydrogenase (Figure 4A,B) and PGC-1α (Figure 4C,D) in these muscles
Prolonged swimming induced a different response in the levels of the metabolites mentioned above, especially in the white, fast-twitched EDL muscle
Summary
Other adverse events of Dex administration are skeletal muscle atrophy and accelerated weight loss [10,11] These negative changes are related to the Dex effect on. Their results show that Dex stimulates mitochondrial biogenesis and that this phenomenon is relatively specific for skeletal muscle [19] Despite these differences in energy metabolism after Dex treatment, skeletal muscle atrophy and changes in mitochondria functions occur as adverse events. Despite the protective effects of physical training on skeletal muscle atrophy induced by Dex, there is no data relating to the effects of Dex on skeletal muscle metabolism under long-term endurance exercise conditions. This study investigates the effect of long-time swimming on blood and skeletal muscle metabolites concentration and skeletal muscle activity of enzymes related to energy metabolism after Dex treatment
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