The effect of lipopolysaccharide extracted from Escherichia coli on total WBCs, granulocytes and on phagocytic activity in female rats

Abstract

The present study was conducted to compare the total white blood cells count, granulocytes and phagocytic activity in female rats immunized with lipopolysaccharide (LPS) extracted from two different isolates of Escherichia coli, one normal isolate from the gut of healthy adult and the other was pathogenic strain isolated from patients suffering from urinary tract infection. The study was included 35 female albino rats divided randomly into seven groups, three groups injected with different concentrations of LPS of normal flora of E.coli 90, 120 and 150 microgram/kilogram body weight respectively and the other three groups were injected with the same concentrations of LPS extracted from pathogenic strain of E.coli, the last group (7th) represented the control group which given normal saline only. The results showed that the LPS from normal isolate causes a significant increase in the total number of leukocytes, granulocytes and phagocytic activity in animals immunized with the concentration of 120 µg/kg compared with the control group, and the concentration of 150 µg/kg of the same extract resulted in a high significant increase in phagocytic activity and the concentration of 90 µg/kg did not stimulate any significant differences in all the variables, while immunization with the extract of pathogenic strain increases the total leukocytes at a concentration of 90 µg/kg only with a significant increases in phagocytic activity at all concentration, and it also did not cause any significant differences in the granulocytes count.