Abstract
<p class="Els-Abstract-text">Some bacteria emits light in the dark. The aim of this research is to find lighting duration and medium composition which produce the most enduring luminescence. The results showed that one of the bacteria isolated from light organ of <em>Loligo duvacelli </em>(D'Orbigny, 1835)<em> </em>squid are able to emit light. The isolation use trisalt solution and cultured into nutrient agar with addition of NaCl emits the longest blue-green light duration under a 1:1 dark-light shift incubation 8.3 d. Meanwhile under a total light, total dark, and in the oven incubator it illuminate for 4.2 d, 3.6 d and 2.6 d, respectively. The combination of beef extract + peptone + a commercial agar + NaCl generate the longest duration of luminescence when incubate in the oven incubator (3,2 d). Meanwhile it illuminated for 2.6 d and 1.7 d on NA + NaCl and NC + NaCl media, respectively, but it failed to illuminate on PCA, NA, and green beans extract + commercial agar + NaCl media. Based on this research we conclude that a commercial agar is potential to replace a technical bacterial agar function.</p><p class="Els-Abstract-text"> </p><div><p class="Els-keywords"><strong>Keywords:</strong> bioluminescence; commercial agar; light treatments; <em>Loligo duvaceli</em> (D'Orbigny, 1835); medium treatment.</p></div>
Highlights
Bioluminescence is a chemical reaction that takes place in an organism and produces detectable light
Bioluminescence typically requires at least three components: a light-emitting organic molecule known as a luciferin; a source of oxygen; and a protein catalyst known as luciferase
Dark treatments were done by putting the culture in a closet and light treatment was done by placing the cultures in a constantly lighted room
Summary
Bioluminescence is a chemical reaction that takes place in an organism and produces detectable light These organisms use a variety of body parts to emit light in different colors and for different purposes. Bioluminescence typically requires at least three components: a light-emitting organic molecule known as a luciferin; a source of oxygen (may be O2, but could be hydrogen peroxide or a similar compound); and a protein catalyst known as luciferase. In some organisms, these three components are bound together in a complex called photoprotein. The aim of this research is to find lighting duration and medium composition which produce the most enduring luminescence
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