Abstract
The impacts of wavelengths in 500–600 nm on plant response and their underlying mechanisms remain elusive and required further investigation. Here, we investigated the effect of light quality on leaf area growth, biomass, pigments content, and net photosynthetic rate (Pn) across three Arabidopsis thaliana accessions, along with changes in transcription, photosynthates content, and antioxidative enzyme activity. Eleven-leaves plants were treated with BL; 450 nm, AL; 595 nm, RL; 650 nm, and FL; 400–700 nm as control. RL significantly increased leaf area growth, biomass, and promoted Pn. BL increased leaf area growth, carotenoid and anthocyanin content. AL significantly reduced leaf area growth and biomass, while Pn remained unaffected. Petiole elongation was further observed across accessions under AL. To explore the underlying mechanisms under AL, expression of key marker genes involved in light-responsive photosynthetic reaction, enzymatic activity of antioxidants, and content of photosynthates were monitored in Col-0 under AL, RL (as contrast), and FL (as control). AL induced transcription of GSH2 and PSBA, while downregulated NPQ1 and FNR2. Photosynthates, including proteins and starches, showed lower content under AL. SOD and APX showed enhanced enzymatic activity under AL. These results provide insight into physiological and photosynthetic responses to light quality, in addition to identifying putative protective-mechanisms that may be induced to cope with lighting-stress in order to enhance plant stress tolerance.
Highlights
Among various environmental factors, light is one of the most important variables affecting photosynthesis as well as plant growth and development [1]
To assess the effect of light quality, 21-days-old plants (11 leave plants) of three A. thaliana accessions Col-0, Est-1, and C24 were randomly divided into groups and treated under narrow-spectrum light (BL, amber light (AL), and red light (RL)), along with fluorescent light (FL) as control, for 5 days at approximately 70 μmol m-2 sec-1 (Fig 1A and 1B)
We examined the antioxidative activity of superoxide dismutase (SOD) and ascorbate peroxidase (APX) enzymes in Col-0 treated under AL, RL, and FL (Fig 5)
Summary
Light is one of the most important variables affecting photosynthesis as well as plant growth and development [1]. Plants require light as an energy source and as a clue to adjust their development to environmental conditions. During photosynthesis, absorbed energy is transferred to the photosynthetic apparatus, which is comprised of Photosystem I (PSI), Photosystem II (PSII), electron transport carriers (cytochrome b6f (cytb6f), plastoquinone (PQ), plastocyanin (PC)), and ATP synthase. The light quality effect on plant physiology, photosynthetic, and stress responses funding received for this study. ML is the author, who received this award. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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