Abstract

The effect of an 8 h light/day or total darkness photoperiod on thein vitro microtuberization of cultivars “Red Pontiac”, “Shepody”, “Kennebec” and “Yukon Gold” was investigated using a medium consisting of Murashige and Skoog salts, vitamins, high sucrose and high benzylamino purine levels and cultured at 16°C for 12 weeks. Percent tuberization of the singlenode leaf cuttings averaged over all 4 cultivars was initially lower, with the 8 h photoperiod at 4 weeks, but later was similar to the total darkness photoperiod at 8 and 12 weeks. Microtubers from all cultivars had a higher mean fresh weight when treated with an 8 h photoperiod as compared with total darkness, though the difference was not significant with “Yukon Gold.” ldRed Pontiac” and “Shepody” produced microtubers that were well over twice the fresh weight of those produced in the total darkness treatment. Delayed leaf senescence, microtuber greening, and nodal rooting were evident with the 8 h light treatment.

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