The effect of light factor on the development of thallus and generative organs in Chara vulgaris L.

Abstract

During long-term axenic culture of Chara continuous illumination (L=24) increases mitotic activity of almost all types of cells. In such conditions only initiation of oogonia is inhibited, leading into a strong predomination of male generative organs. Prolonged darkness (L:D=1:23) exerts a mitodepressing effect. Oogonia and antheridia are especially susceptible to the reduction of the light period. Modifications of the elongating growth in various photoperiods are different in the polyploid regions of the vegetative thallus and in haploid cells of the antheridial filaments. Segments of both axial internodes and lateral pleuridia increase their dimensions at L:D=1:23, whereas at L=24 their growth is significantly inhibited. Different reaction is noted in the cells of antheridial filaments: at L=24 they are about 10% longer than in the control (L:D=14:10). The duration of the antheridium aevelopment, from the stage of unicellular filaments to the moment of antheridium opening, is 1.5 days shorter at L=24 as compared with the control. This shortening includes proportionally both the period of divisions within antheridial filaments and the period of spermatozoid differentiation.