Abstract
Somatic embryogenesis and organogenesis of lilies have not been investigated in detail before, although tissue culture methods have been applied to the propagation for decades. We investigated the effects of light and different auxins on regeneration in lily (Lilium regale Wil.) callus at a morphologic and anatomic level. In addition to solid medium, cell suspension cultures were established for somatic embryogenesis. Our results showed a high regeneration capacity for lily callus and a rate of somatic embryogenesis that reached 10% of all shoots formed. Differentiation was promoted when the callus was kept in the dark during propagation and transferred into light only prior to regeneration. Naphtalene acetic acid was superior compared with 2,4‐D in both the regeneration and proliferation of callus of this species, as 2,4‐D resulted in malformed shoots and necrosis, and it inhibited the regeneration in general. Morphological investigations shared differences in the structure of bulbets arising from the different regeneration processes. Callus in suspension showed high regeneration capacity, and organogenic and embryogenic sites and somatic proembryos were detected.
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