The effect of LED light spectra on antioxidant system by thermal stress in goldfish, Carassius auratus

Abstract

We investigated the effects of the different light emitting diodes (LEDs; red, green, blue, and mixed purple) on oxidative stress by measuring the expression and activities of antioxidant enzymes, plasma H2O2, lipid peroxidation (LPO), aspartate aminotransferase (AspAT), alanine aminotransferase (AlaAT) levels, and melatonin during thermal stress (low-water temperature, 22 → 14°C; high-water temperature, 22 → 30°C) on goldfish, Carassius auratus. The expression and activity of the antioxidant enzymes and levels of H2O2, LPO, AspAT/AlaAT, and melatonin were significantly higher after the fish were exposed to low-/high-water temperature environments. Furthermore, in green and blue lights, the expression of antioxidant enzymes and mRNA of heat shock protein 70, and the activity of the antioxidant enzymes and plasma H2O2, LPO, and melatonin levels were significantly lower than those under other light spectra. The results indicated that low-/high-water temperature conditions induced oxidative stress in goldfish and green and blue LEDs inhibit oxidative stress.