The effect of Lactobacillus plantarum hydrolysates promoting VEGF production on vascular growth and hair growth of C57BL/6 mice

Young Min Woo,Chun-Ri Li,Sang-Hyeon Lee,Mee Young Ahn,Eun Sol Jo,Andre Kim,Jong Myung Ha,Jae-Suk Choi,Min Young Jo,Young-Ho Lee,Ok Ju Kim

doi:10.1186/s40543-019-0178-0

Abstract

PurposeAngiogenesis is critical in various biological processes, such as blood vessel growth, fetal differentiation, wound healing, and organ regeneration. Various growth factors have been associated with vascular regeneration, including insulin-like growth factor 1 (IGF-1), transforming growth factor-β (TGF-β), and basic fibroblast growth factor (bFGF). One of the most important mediators of vascular regeneration is vascular endothelial growth factor (VEGF). VEGF is known to increase vascular permeability, induce the proliferation of endothelial cells, and stimulate capillary formation in vivo, which are core angiogenic functions.MethodsThe hydrolysates of lactic acid bacteria were produced by hydrolyzing Lactobacillus plantarum with proteases, treated with MG-63 osteoblasts, and screened to obtain samples with an excellent VEGF production effect. These samples were applied to human dermal papilla cells (hDPC) to examine the correlation between cell growth and VEGF secretion. Furthermore, the hair growth rate was measured in hair growth experiments using C57BL/6 male mice.ResultsThe hydrolysates of the lactic acid bacteria produced in this study produced hair growth superior to the growth obtained with 5% minoxidil in hair growth experiments using C57BL/6 male mice.ConclusionsThis study aims to develop a material for application to the scalp that promotes angiogenesis in the scalp and facilitates the exchange of nutrients and wastes in the follicles to promote hair growth.

Highlights

IntroductionOne of the most important mediators of vascular regeneration is vascular endothelial growth factor (VEGF)
Hair loss treatments applied directly to the scalp that are currently researched include helping hair growth by nutrient supply and the release of harmful elements through vasodilation or blood circulation promotion, reducing stress on the hair follicle by inhibiting the generation of reactive oxygen species (ROS) or nitric oxide (NO) that cause inflammation, reducing the risk factorsWoo et al Journal of Analytical Science and Technology (2019) 10:18One of the most important mediators of vascular regeneration is vascular endothelial growth factor (VEGF)
The hydrolysates of the lactic acid bacteria produced in this study produced hair growth superior to the growth obtained with 5% minoxidil in hair growth experiments using C57BL/6 male mice

Summary

Introduction

One of the most important mediators of vascular regeneration is vascular endothelial growth factor (VEGF). VEGF is known to increase vascular permeability, induce the proliferation of endothelial cells, and stimulate capillary formation in vivo, which are core angiogenic functions (Miele et al 2000; Yeh et al 1999). There are reports that VEGF is secreted in dermal papilla cells. In light of the fact that there are rich capillaries in the papilla during hair growth, but that they disappear during the transition into the resting period, it is thought that the growth of blood vessels is associated with hair growth and that VEGF is involved in hair growth. VEGF has been reported to reduce hair loss by promoting blood circulation and the formation of new blood vessels around follicles (Back et al 1999)

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