Abstract
SummaryThiol groups of rat-liver nuclei, mitochondria, lysosomes, and microsomes are destroyed by irradiation in vitro with electron doses of 5–50 kilorads. Significant destruction of thiol content occurs in all sub-cellular fractions immediately after a dose of 5 kilorads, the percentage of -SH groups destroyed being 9·5 per cent in the nuclei, 23 per cent in the lysosomes, 9·1 per cent in the mitochondria and 2·8 per cent in the microsomes. The percentage of thiol groups destroyed increased with radiation dose in all fractions. Incubation in air or oxygen at 4°c, 20°c or 37°c after irradiation causes further destruction of sulphydryl groups, but this does not occur after incubation in nitrogen.It is suggested that the destruction of -SH groups is a result of oxidation by free radicals produced in the aqueous phase and is not a result of oxidation by lipid peroxides.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: International journal of radiation biology and related studies in physics, chemistry, and medicine
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.