Abstract

When mealy bug testis cells are placed in an isotonic salt solution, a large mass of heterochromatic chromosomes is visible. As the ionic strength is lowered, these chromosomes decondense and can no longer be distinguished from the euchromatic chromosomes. This change is reversed by returning the cells to isotonic medium. Labeling experiments showed that this recondensation process is specific in the sense that it is the original heterochromatic chromosomes which recondense. In isotonic salt solutions, the heterochromatic chromosomes synthesize little or no RNA as compared to the euchromatic chromosomes. In hypotonic solution, cells show a general decrease in RNA synthesis, but they exhibit the same pattern of RNA synthesis as cells in isotonic solution. Therefore, it is concluded that this ion-mediated decondensation of chromosomes does not increase their capacity to synthesize RNA.

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