Abstract
The inactivation of a canine adenovirus hemagglutinin and infectivity at 56 °C at varying times up to 1 h has been studied in the presence of various ions and concentrations of ions.Under conditions of uncontrolled pH 1–2 M NaCl and MgCl2 both stabilized the virus hemagglutinin and infectivity. Lower concentrations of these salts as well as 0.04 M phosphate, Hank's balanced salt solution, and minimal Eagle's medium had little stabilizing effect. Molar concentrations of phosphate and Tris butter were also found to stabilize the virus hemagglutinin.The pH adjustment of the suspending salt solution was found to be important since high concentrations of salts such as MgCl2 are quite acidic. Both 1 M MgCl2 and 1 M NaCl stabilized the virus in the pH range of 4–6, but inactivation was rapid at pH 3 and less rapid at pH 7. Ion concentrations lower than 1 M markedly reduced the hemagglutinin stabilization at optimun pH.
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