Abstract
It is shown that callose synthesis in the <em>Oenothera biennis </em>pollen tube is regulated by the endogenous Ca<sup>2+</sup> level. Calcium antagonists reduced the amount of callose in the wall above the tip of the pollen tube (Verapamil - calcium channels blocker) and at the tube tip after stopping tube growth (La<sup>3+</sup> - a Ca<sup>2+</sup> substitute). Ruthenium red and ionophore A 23187, which raise the Ca 21 level in the cytoplasm, induced callose synthesis at the tip of pollen tube.
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