The effect of intestinal ischemia on plasma thiol/disulphide homeostasis in an experimental study

Abstract

Aim: To investigate the effects of acute intestinal ischemia on plasma thiol/disulphide homeostasis (TDSH), which has been investigated in a limited number of studies in the related literature. Methods: Twenty-four rats were randomized into control (operation without ischemia, GIS), and ischemia groups (GII-60, GIII-180). For ischemia, the superior mesenteric artery was sutured and the rats were exposed to 60 and 180 minutes of intestinal ischemia, respectively. Plasma TDSH was measured in blood samples collected at the end of the ischemia, and the pathology of ileum segments resected was evaluated. Results: The experimental ischemic conditions provided were confirmed by the total histopathological scoring system statistically. The levels of serum human albumin and ischemia modified albumin (IMA) in groups were detected in quite a close range of each other. There was no found a statistically significant difference for IMA between groups (p>0.05). The alternations on the levels of plasma TDSH parameters were observed in the study. According to ischemic conditions, the thiol/disulfide ratio fluctuations were detected in the plasma TDSH. The native thiol and total thiol levels seem to have decreased according to ischemia; no statistical difference was detected. In addition, the disulfide levels increasing according to ischemia either was not found significant statistically (p>0.05). Conclusion: Although this study showed the oxidative balance in intestinal ischemia had affected plasma TDSH, also it revealed that intestinal ischemia didn't create a statistically significant difference between plasma TDSH components.